石墨烯-聚乙烯醇-钯复合物的制备及在克林沙星检测中的应用

摘要

用石墨烯-聚乙烯醇-钯修饰电极（GN-PVA-Pd／GCE）测定水产品中的克林沙星（CFN）。研究了CFN在GN-PVA-Pd／GCE上的电化学行为，并探讨了示差脉冲伏安法检测CFN的可行性。结果表明，在pH＝10、扫速＝100mV·s－1的条件下，GN-PVA-Pd／GCE修饰电极对CFN的响应效果最好，检测CFN的线性范围分为两段，在0.09～5.4μM CFN浓度区间，峰电流与CFN浓度的线性关系为I （μA）＝0.998 CCFN（μM）＋0.034；在5.4～72μM CFN浓度区间，线性关系为I （μA）＝0.207CCFN（μM）＋4.154。检出限为0.03μM（信噪比S／N＝3）。GN-PVA-Pd／GCE用于检测水产品中CFN，加标回收率为96.7％～106.6％，RSD为2.8％～3.9％。%Graphene-polyvinyl alcohol-Pd(GN-PVA-Pd)nanocomposite was synthesized and applied to the determination of clina-floxacin(CFN). The electrochemical behavior of CFN on the nanocomposite was investigated and a differential pulse voltammetry method was developed for the determination of CFN. The results showed that the peak current increased with the CFN concentration in the range of 0. 09~5. 4μM with a calibration equation of I (μA)=0. 998CCFN+0. 034(μM)and in the range of 5. 4~72μM with a calibration equation of I (μA)=0. 207CCFN+4. 154(μM)under the conditions of pH 10 and scan rate 100mV·s-1 . The de-tection limit was estimated to be 0. 03μM(S/N=3). The modified electrode has been applied to the determination of CFN in aquat-ic product samples with the recoveries of 96. 7% ~106. 6%.