硅表面CS/BSA复合微图形的制备及表征

摘要

Chitosan/Bovine serum albumin (CS/BSA) composite micropatterns were successfully fabricated on silicon surfaces by micro-transfer molding method. The micropatterned CS/BSA composite films were studied by optical microscopy and fluorescent microscopy. A quantitative test was carried out to characterize the antibacterial properties of the CS/BSA composite films. The results show that the micropatterned CS/BSA composite films are effectively against E.coli and Staphylococcus albus. MC3T3-E cell culture test indicated that micropatterned CS/BSA composite films could strongly direct the orientation of cells. Moreover, the micropatterned CS/BSA composite films could promote the cell adhesion, spreading, proliferation and differentiation. Thus, the micropatterned CS/BSA composite film fabricated by micro-transfer molding method has good antibacterial properties and cell compatibility.%采用微加工方法在硅表面成功地制备出壳聚糖/牛血清白蛋白(CS/BSA)复合微图形. 借助光镜和荧光显微镜对复合微图形进行形貌分析. 利用大肠杆菌和白色葡萄球菌定量考察了CS/BSA复合微图形的抗菌性能, 结果表明, 硅表面沟槽交叉状CS/BSA复合微图形对两种细菌都具有较好的抗菌效果. 通过MC3T3-E成骨细胞培养考察了复合微图形的细胞相容性, 结果表明, CS/BSA复合微图形对于细胞的生长方向具有较强的诱导性, 可促进细胞在材料表面的黏附、铺展及增殖分化. 结果表明, 采用微转移模塑法制备的CS/BSA复合微图形具有较好的抗菌性和细胞相容性.