基于UPLC-Q-Orbitrap MS/MS研究人参皂苷r在发酵过程中的生物转化

摘要

应用超高效液相色谱-四极杆-静电场轨道阱串联质谱(UPLC-Q-Orbitrap MS/MS)联用技术分析鉴别人参发酵前后的皂苷类化合物,研究了整体人参皂苷在自然发酵过程中的生物转化规律.通过鉴定45种人参皂苷,比较了生晒参和发酵人参中人参皂苷种类及含量的差异,在发酵人参中检测到20(S)-Rg3和20(R)-Rg3,20(S)-Rh1和20(R)-Rh1等异构体人参皂苷,其中10余种人参皂苷在自然人参状态下不存在或未被检出.本研究对于人参发酵前后的化学成分变化进行了高通量的精准分析,对同类天然产物发酵研究具有借鉴意义,可指导人参炮制品生晒参和发酵人参的安全有效使用.%This work aims to analyze and compare the ginsenosides contents from white ginseng and fermented black ginseng(FBG) using UPLC-Q-Orbitrap MS / MS(ultra performance liquid chromatography coupled with quadrupole orbitrap tandem mass spectrometry). FBG was processed by spontaneous short-term fermentation, along with the biotransformation of ginsenosides. The samples were separated by liquid chromatography using a Thermo Syncronis C18 column at 30 ℃. Moreover, 0. 1％(volume fraction) formic acid and acetonitrile were used as mobile phases A and B, respectively. The flow rate was 0. 2 mL/ min, and the injected sample volume was 0. 5 μL. By the use of the UPLC-Q-Orbitrap MS / MS, 52 kinds of ginsenosides were identified. The contents of main ginsenosides, especially in malonylated ginsenosides, were decreased, because of hydrolyzed. The method of spontaneous short-term fermentationdid doesn' t require any additives, and provides the theoretical basis for spontaneous short-term fermentprocessed ginseng.