FAAS法间接测定维生素片中维生素B1的含量

摘要

建立了火焰原子吸收光谱(FAAS)法间接测定维生素B片中的维生素B1含量的方法.用过量的Ag+与硫胺素盐酸盐的Cl-反应,产生AgCl沉淀,用火焰原子分光光度计测量上清液中剩余Ag+含量进而计算出样品中维生素B1的含量.方法回收率在98％～102％之间,相对标准偏差(RSD％)在1.05％～1.87％之间,方法操作简便,快速,重现性好.%An indirect method for the determination of vitamin B1 by flame atomic absorption spectrometry (FAAS).Excess Ag+ reacted with Cl- exists in thiamine hydrochloride to form AgCl, which was separated by centrifuge. Vitamin B1 was determined indirectly by determination of Ag+ in supernatant fluid. The average recoveries were between 98% and 102% ,and the relative standard deviations (RSD%) were 1.05%-1.87%. The results showed that the method was sensitive, accurate, convenient and quick, which can be used to detect Vitamin Bj in vitamin tablets.