建立了高效液相色谱-四极杆飞行时间串联质谱快速检测饮料中糖精钠、甜蜜素、安赛蜜、阿斯巴甜、纽甜、三氯蔗糖6种人工合成甜味剂的方法。样品经水提取，采用C18色谱柱，以甲醇和0.1%甲酸-10 mmol/L甲酸铵溶液为流动相，梯度洗脱，四极杆飞行时间串联质谱电喷雾负离子模式检测。各化合物在0.02～2.0 mg/L范围内均呈现良好的线性关系，相关系数均大于0.998。样品平均添加回收率为63.0%～113.2%，测定结果的相对标准偏差均小于9.6%(n=5)。该方法简便快捷，选择性好，灵敏度高，可满足国内外现行法规的限量要求。%A new method was established for the determination of 6 synthetic sweeteners (sodium saccharin, cyclamate, acesulfame-K, aspartame, neotame, sucralose) in beverage, by using high performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-Q TOF MS). The samples were extracted by water and measured directly by HPLC-Q TOF MS with electrospray ionization in negative mode. The compounds were separated by a C18 column with methyl alcohol and 0.1% formic acid-10 mmol/L ammonium formate solution as the mobile phase. A detailed fragmentation study for 6 synthentic sweeteners was carried out by time of flight. The 6 compounds behave linearly in the range of 0.02-2.0 mg/L with the correlation coefficient of more than 0.998. The recoveries were 63.0%-113.2%, with coefficients of variation below 9.6%(n=5). The method is suitable for routine qualitative and quantitative analyses of synthetic sweeteners in beverage.
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