紫外可见分光光度法测定酶解桑葚酒中多糖含量

摘要

采用紫外可见分光光度法测定酶解桑葚酒中的多糖含量。向酶解桑葚酒中加入硫酸，使样品中的多糖水解成单糖，并迅速脱水生成糖醛衍生物，加入苯酚作为显色剂，检测波长为490 nm，标准工作曲线线性方程为y=0.0568x–0.035，相关系数r=0.9994。测定结果的相对标准偏差为0.03%（n=5），平均加标回收率为98.69%。该方法用于检测酶解桑葚酒中多糖含量，简便、快速。%The content of polysaccharide in Mulberry wine made by enzymatic method was determined by UV spectrometry. Sulphuric acid was added in Mulberry wine sample, and polysaccharide in the sample was hydrolyzed to monosaccharide which was dehydrated to geberate furfural derivatives, then phenol was added as chromogenic agent, the detection wave length was 490 nm. The standard curve regression equation wasy=0.056 8x–0.035 with the correlation coefficient of 0.999 4. The relative standard deviation of the detection results was 0.03%(n=5), and the average recovery was 98.69%. This method is simple and fast, it can be used to determine polysaccharide in Mulberry wine.