酶法酯交换催化合成中/长链结构甘三酯原料药工艺研究

摘要

该文以中碳链甘三酯和大豆油为原料，对酶法酯交换催化合成中/长链结构甘三酯的工艺进行研究。系统研究了脂肪酶的种类、脂肪酶添加量、反应温度和反应时间四个因素对酯交换反应程度的影响，结果表明，选用脂肪酶TL IM，脂肪酶添加量5%（以油重计），反应温度65℃，反应时间30 min时酯交换反应即达到平衡状态，中/长链结构甘三酯的含量为73.73%，符合结构甘油三酯进口药品注册标准的含量要求。正交极差分析结果显示，选定脂肪酶的添加量是影响酯交换反应程度最重要的因素，反应温度次之，反应时间最小。%Basedon themedium-chain triglycerides and soybeanoil as rawmaterial, the enzymatic transesterificationcatalyzed synthesis themedium/long-chain structure triglycerides process was studied in the paper.The influencesof the kindsof lipase, lipase load, reaction temperature and timeon the degreeof transesterification were systematically studied, and the results showed that theLipozyme TL IM, 5% lipase/oil(w/w), reaction temperature 65℃, reaction time 30min were theoptimal conditions and thecontentofmedium/long-chain structureof triglycerides was 73.73%, which was in line with the imported drug registration standard requirementsof structure triglycerides. We also found that the amountofLipozymeTL RM was themost important factor for the degreeof transesterification by theorthogonal range analysis, followed by the reaction temperature and reaction time.