四物汤抗环磷酰胺诱变作用的研究

摘要

目的:研究四物汤抗环磷酰胺的诱变作用.方法:小鼠骨髓细胞实验中,设四物汤高、中、低剂量组[分别为9.36、4.68、2.34 mg/(kg·d)],环磷酰胺(cyclophosphamide,CP)阳性对照组和空白对照组,四物汤各组以不同浓度药液给小鼠连续灌胃7d,阳性对照组和空白对照组灌服等量生理盐水.于第7天灌胃后,四物汤各组和阳性对照组小鼠腹腔注射CP 20 mg/kg,24 h后处死动物,取股骨骨髓细胞制备微核标本和染色体标本.人外周血淋巴细胞实验中,四物汤设高、中、低剂量[分别为3.36、1.68、0.84 g/(kg·d)],以家兔含药血清的形式加入淋巴细胞培养液中,四物汤各组和阳性对照组均在培养液中加入10-5 mg/mL的CP,空白对照组加入等量生理盐水.培养72 h后收获细胞,制备微核标本和染色体标本.在光镜下观察、计数含微核及染色体畸变的细胞数,计算微核率及染色体畸变率.结果:四物汤3个剂量组在上述测试系统中,与阳性对照组比较,微核率及染色体畸变率均明显降低,差异有统计学意义(P＜0.01)；与空白对照组比较,微核率及染色体畸变率差异均无统计学意义(P＞0.05).结论:在本实验条件下,四物汤有明显的抗环磷酰胺诱变的作用.%OBJECTIVE: To study the anti-mutagenic effects of Siwu decoction. METHODS: Four test methods were used; Mouse bone marrow cell micronucleus test, chromosomal aberrations in mouse bone marrow cells test, micronucleus test and chromosomal aberration test in human peripheral blood lymphocytes in vitro. Five groups were included in each test method, including blank control group (physiological saline group), positive control group (cyclophosphamide group) and different doses of Siwu decoction groups. High dose group Siwu decoction was 9.36 mg/(g ·d), middle dose group 4.68 mg/(g·d), and low dose group 2.34 mg/(g·d). In blood lymphocytes in vitro test, Siwu decoction was added to culture medium in the form of serum. To prepare serum in rabbits, high dose was 3.36 g/(kg·d), middle dose 1.68 g/ (kg·d) and low dose 0.84 g/(g·d). Cells contained micronucleus and chromosomal aberrations in specimens were examined and counted. RESULTS: Compared with positive control group, the rate of micronucleus and chromosomal aberrations in three different Siwu decoction dose groups were obviously lowered (P<0.01). Compared with blank control group, the rate of micronucleus and chromosomal aberrations in three different Siwu decoction dose groups were higher(P>0.05). CONCLUSION: In the range of this experiment, Siwu decoction showed obvious anti-mutagenic effects.