三氯乙烯对L-02肝细胞中SET相互作用蛋白eEF1A1、eEF1A2和DDB1 mRNA表达水平的影响

摘要

OBJECTIVE:Toresearch the effects oftrichloroethylene on the RNA expressions ofSET-associated proteins including eEF1A1,eEF1A2 and DDB1 in L-02 cells.METHODS:L-02 cells,being in the logarithmic growth phase,were t reated w ith d ifferent c oncentrations (1. 0, 2. 0, 4. 0, 8. 0mmol/L) oft richloroethylene for 2 4 h ,andD MSO w ere used as control. The mRNA levels ofeEF1A1, eEF1A2 and DDB1 were analyzed with qPCR.RESULTS:The results showed that mRNA levels ofDDB1 and eEF1A2 were significantly up-regulated under low concentration oftrichloroethylene (1.0 mmol/L) and were significantly down-regulated with increasing concentrations oftrichloroethylene. The mRNA levels ofeEF1A1 were significantly up-regulated with increasing oftrichloroethylene concentration.CONCLUSION:Exposure oftrichloroethylene c ould a lter m RNA e xpression ofS ET-associated p roteins i n L -02 c ells.%目的：法：取对数生长期的L-02肝细胞，暴露于不同浓度TCE(1.0、2.0、4.0、8.0 mmol/L)中，以DMSO为溶剂对照。24 h后，采用实时荧光定量PCR检测eEF1A1、eEF1A2和DDB1 mRNA表达水平。结果：与对照组相比，不同浓度的TCE均能够使L-02肝细胞eEF1A1、eEF1A2和DDB1 mRNA表达水平发生明显变化。其中，DDB1和eEF1A2 mRNA表达水平在低浓度(1.0 mmol/L)TCE暴露下显著升高，而随着TCE浓度的升高其mRNA表达水平显著降低(P<0.05或<0.01)。eEF1A1 mRNA表达水平随着TCE浓度的增加而升高(P<0.05)。结论：TCE染毒可诱发L-02肝细胞中SET相互作用蛋白的mRNA表达水平发生改变。探讨人L-02肝细胞经三氯乙烯(TCE)染毒后SET相互作用蛋白eEF1A1、eEF1A2和DDB1 mRNA表达水平的变化。方P