cDNA-AFLP和cDNA-SRAP技术在植物基因差异表达上的应用及其分析比较

摘要

对cDNA-AFLP和cDNA-SRAP技术在植物上的应用研究进行了介绍,指出cDNA-AFLP和cDNA-SRAP技术已逐渐成为基因表达研究的重要工具,是构建转录图谱、定位目标性状位点、研究差异基因表达等研究的主要手段,具有广泛广阔的应用前景.但是cDNA-AFLP和cDNA-SRAP技术均具有一定的缺限,主要表现在获得的差异片段还不够全面丰富.cDNA-AFLP可以扩增到扩增较多的带,但试验程序比较繁琐、昂贵而且耗时较长、操作较困难;而cDNA-SRAP相对比较简便、便宜而耗时较短,较易操作.因而将cDNA-AFLP与cDNA-SRAP技术结合起来,可望得到更全面丰富的差异片段,从而在植物基因差异表达、新基因发现、抗逆性分子机理研究等方面发挥更大的作用.%The applicationof cDNA-AFLP and cDNA-SRAP in plant species was reviewed in this paper. Both cDNA-AFLP and cDNA-SRAP techniques have become important approaches to study gene expressions,especially in constructing transcriptions! maps, mapping objective traits aim characters and gene differential expressions in plant. But both methods have certain limitations, and the main problem is the obtained differential fragments are not completely satisfied. In comparison, the protocol of cDNA-AFLP seems complicated and costly,which could amplify more polymorphic bends. The protocol of cDNA-SRAP is simpler and cheaper than that of cD-NA-AFLP. Combination of both cDNA-AFLP and cDNA-SRAP techniques may obtain longer and more complete differential fragments than each individuals, thus could play great roles in studying gene differential expressions, finding new genes and investigating the molecular mechanisms of stress resistance in plants.