高分辨率熔解曲线及其应用

摘要

饱和荧光染料、未标记探针与实时荧光PCR结合产生的高分辨率熔解曲线(HRM)是一种新的实时定量技术,在检测速度、灵敏度和准确性上具有突出的优点,近几年来在突变扫描、DNA甲基化和基因分型等医学检测中发展迅速.就HRM的原理、应用以及在HRM基础上发展起来的未标记探针(unlabled probe)HMR、弹回探针(snap probe)HMR技术作一介绍.%High resolution Melting is a new real-time PCR technology, which combines saturated dyes, unlabled probe and real-time PCR. The advantage of HRM is rapid detection, high sensitive and accurate. Recently, the application of HRM have involved in medical mutation scanning and genotyping. The aim was to introduce the principle, application of HRM, unlabled probe HMR and snap probe HMR.