低聚半乳糖对肠道益生菌产胞外多糖作用的研究

摘要

旨在研究低聚半乳糖对肠道益生菌产胞外多糖的作用。以低聚半乳糖、葡萄糖、半乳糖和乳糖4种碳源作比较，分别从碳源的利用、胞外多糖的产量和种类及其对有害菌的黏附作用进行研究。结果显示，低聚半乳糖不仅能促进长双歧杆菌和植物乳杆菌的增殖，而且有利于胞外多糖的产生，其含量分别为208.78μg/mL和192.78μg/mL，产生的胞外多糖种类较其他3种碳源更多，对肠道有害菌大肠杆菌具有明显黏附作用。结果证明，与其他3种碳源相比，低聚半乳糖能促进植物乳杆菌和两歧双歧杆菌生成更多的胞外多糖。%The aim of the study is to investigate the effects of galactooligosaccharide on the yield of exopolysaccharides produced by intestinal probiotics. Using galactooligosaccharide, glucose, galactose and lactose as carbon sources, the utilization of carbon sources, the yield and varieties of exopolysaccharides and the adhesion of harmful bacteria werestudied.Results indicated that galactooligosaccharide not only promoted reproduction ofBifidobacterium longumand Lactobacillus plantarum, but also improved the yield of exopolysaccharides with 208.78μg/mL and 192.78μg/mL respectively. Varieties of exopolysaccharides produced while using galactooligosaccharide as carbon source were more than other 3 ones, and the adhesion to harmful bacteria such asEscherichia coliwas obvious.It is proved that galactooligosaccharide could promoteB. longumand L. plantarum to produce more exopolysaccharides compared with the other 3 carbon sources.