microRNA-483和microRNA-486在克隆和转fat-1基因牛组织中的表达

摘要

The expression levels of microRNA-483 and microRNA-486 in heart,liver,spleen,lung,kidney,placenta, cotyledons,endometrium from normal,cloned,and transgenic bovine were measured by real time quantitative PCR. The results showed that microRNA-483 and microRNA-486 were expressed in all tissues of normal,cloned and fat-1-transgenic bovine;significantly higher in the heart than other tissues. However,the expression levels of microRNA-483 and microRNA-486 in transgenic bovine were lower than normal one. Further,the expressions of microRNA-483 and microRNA-486 varied in different tissues and high in the heart,indicating that the expressions of microRNA-483 and microRNA-486 may be correlated with pathophysiological process of myocardial hypertrophy and myocardial infarction.%利用荧光定量PCR比较正常受精牛、克隆牛和转基因牛的心、肝、脾、肺、肾、胎盘、子叶、子宫内膜中miR-483和miR-486的表达水平。结果显示，miR-483和miR-486在正常受精牛、克隆牛和转fat-1基因牛的组织中均有表达，其中在心脏中表达量显著高于其他组织。而转fat-1基因牛心脏中miR-483和miR-486表达量均低于正常牛。miR-483和miR-486在不同组织中表达量存在一定差异，在心脏中呈现高表达，提示miR-483和miR-486表达降低可能与心肌肥大、心肌梗死等病理生理过程有关。