稳定碳同位素技术在苹果醋饮料掺假鉴别中的应用初探

摘要

Stable isotopic techniques play am important role in food authenticity evaluation. In this paper,the stable carbon isotope technologies (δ13CVPDB)were used to detect the adulteration of apple vinegar. On the based of δ13CVPDB character of sugar of apple,the distribution of δ13CVPDB value of acetic acid of apple vinegar was put forward. Results showed that,apple tree was a classic C3 plant with sugar δ13CVPDB value ranges from -27.11‰~-23.75‰ for the limited samples,and the δ13CVPDB value of acetic acid showed a positive correlation while pure acetic acid derived from fermentation of corn. 20 commercial apple vinegar samples were collected,and δ13CVPDB value of acetic acid indicated that 6 samples were adulterated with the illegal addition of corn acetic acid. This paper demonstrated a new method to discriminate real apple vinegar from fraud products. This finding and its possible application is a great significance for upgrading the technical level of China National Standard of vinegar and affiliate the imports and exports of vinegar.%稳定同位素技术在食品真实鉴别中具有重要应用价值，本文借助稳定碳同位素技术研究了苹果醋饮料的掺假检测方法。基于苹果的糖中碳同位素啄13CVPDB数据，提出了苹果醋饮料的乙酸啄13CVPDB值范围。研究表明，苹果具有典型的C3植物的碳同位素分布特征，苹果醋中掺入玉米原料生产的冰乙酸后乙酸啄13CVPDB值与冰乙酸掺入量呈线性正相关关系。采集了20个市售苹果醋饮料，检出6个掺有玉米原料冰乙酸的阳性样品。该技术可用于苹果醋的真假鉴别，填补了稳定同位素技术在我国果醋饮料真伪鉴别中的应用空白，对实现我国果醋标准与国际接轨、促进果醋的进出口和保护消费者合法权益具有重要意义。