NOGGIN基因过表达对MDA-MB-231乳腺癌细胞系增殖和侵袭的影响及机制

摘要

目的 基因对乳腺癌细胞MDA-MB-231增殖、侵袭能力的影响,并初步探讨其机制.方法 表达NOGGIN方法以NOGGIN重组腺病毒感染乳腺癌细胞系MDA-MB-231,MTT法检测细胞增殖能力,划痕修复实验及Transwell细胞侵袭实验检测其运动和侵袭能力,定量RT-PCR和总蛋白Western blot检测CXCR4和MMP1的表达.结果 过表达NOGGIN基因的MDA-MB-231细胞增殖能力增强(P＜0.05)；细胞划痕愈合延迟；NOGGIN组穿膜细胞数为79±4,显著低于空病毒组的135±7(P ＜0.05).过表达NOGGIN后,细胞中CXCR4和MMP1的表达下调(P＜0.05).结论 NOGGIN蛋白可抑制乳腺癌细胞MDA-MB-231的运动和迁移.其机制可能与下调CXCR4和MMP1的表达相关.%Objective To investigate the effection and mechanism of NOGGIN protein in proliferation and invasion of human breast cancer cell MDA-MB-231. Methods MDA-MB-231 cells were infected with recombinant NOGGIN adenovirus. MTT, Wound-healing experiment and Matrigel invasion assays were performed to examine the change of proliferation, movement and invasion. The expression of MMP1 and CXCR4 was evaluated by real-time PCR and Western blot. ResultsThe growth of MDA-MB-231 cells infected with Ad-NOGGIN was faster than that of the control (P < 0. 05), while scratch repair time extended. Transwell cell invasion assay showed that the invasive ability of MDA-MB-231 cell was inhibited in NOGGIN group (79 ±4), compared with GFP group ( 135 ±7) (P<0. 05). Using real-time PCR and western blot, the expression of CXCR4 and MMP1 was confirmed to decrease. Conclusion NOGGIN protein can enhance the proliferation of MDA-MB-231 cell, and inhibit its invasion and metastases. Maybe the cancer-related gene CXCR4 and MMP1 are involved.