苹果酸舒尼替尼抑制膀胱癌T24细胞系的体外增殖

摘要

Objective The study is aimed at determining inhibition effect on human bladder cancer line T24 by sunitinb in vitro. Methods T24 bladder cells line were treated by 0.625, 1.25, 2.5,5,10 and 20 μmol/L sunibinib in 24, 48 and 72 h. The cytotoxic activity was analyzed by MTT. Results Sunitinib inhibited T24 cell proliferation in dose-dependent manner. When the plasma drug concentration exceeded up to 5 μmol/L, the inhibitory rate of Sunibinib was more than Cisplatin (P ＜0. 05). Inhibitory rate of Sunibinib increased with the extension of incubation time, which showed no significant difference to Cisplatin. Conclusion Sunitinib inhibited bladder cell line T24 proliferation in dose-dependent and time dependent manner.%目的 探讨苹果酸舒尼替尼对人膀胱癌T24细胞系体外增殖的影响.方法 取人膀胱癌T24细胞系悬液,分别加入浓度为0.625、1.25、2.5、5、10和20 μmol/L的苹果酸舒尼替尼,分别于24、48及72 h进行处理,用细胞毒抑制实验(MTT法)检测药物对其生长的影响.结果 苹果酸舒尼替尼可浓度依赖地抑制人膀胱癌细胞系T24细胞增殖,当药物浓度>5μmol/L时,苹果酸舒尼替尼与顺铂比较,对T24细胞系具有更强的抑制作用(P<0.05).这种抑制作用随着约物培育时间延长(24、48,72 h)面增强,表现为IC50值显著降低,但其抑制能力与顺铂比较无显著差别.结论 苹果酸舒尼替尼可呈浓度和时间依赖地抑制膀胱癌T24细胞系增殖.