乳腺癌中RIN1的表达及临床意义

摘要

目的 研究RIN1在乳腺癌中的表达,分析它与乳腺癌预后的关系.方法 用免疫组化SP法检测85例乳腺癌和20例乳腺良性病变组织中RIN1的表达,并对85例乳腺癌病例进行随访；用real-time PCR和Western blot 法检测RIN1在20例乳腺癌和癌旁组织中的表达.Kaplan-Meier分析生存结果.结果 85例乳腺癌标本中RIN1有不同程度表达,其中44例高表达,乳腺癌中RIN1的表达明显高于非癌组织(P＜0.05)；RIN1高表达与肿瘤最大直径、组织学分级、肿瘤家族史成正相关(P＜0.05)；RIN1高表达患者的平均生存时间低于RIN1低表达患者(P＜0.05)；RIN1高表达影响肿瘤无病生存率(P＜0.05).20例标本中RIN1的mRNA及蛋白在乳腺癌的表达明显高于癌旁组织(P＜0.05).结论 RIN1影响乳腺癌的发生发展,其高表达RIN1可能成为判断乳腺癌预后的一个标志物.%Objective To investigate the expression of RIN1 in breast cancer and to analyze its association with the prognosis.Methods To analyze the tissues from 85 breast cancer and 20 benign breast disease patients by immunhistochemical techniques,and followed up the 85 cases of breast cancer; RIN1 in 20 cases of breast cancer and adjacent tissues' expressions by Real Time PCR and Western blot assay.Kaplan-Meier survival analyzed the average survival time.Results A varying degrees of RIN1 were expressed in 85 cases of breast cancer,high expression in 44 cases,RIN1 was highly expressed in breast cancer than in non-cancer tissues(P ＜ 0.05);The expression of RIN1 was significantly associated with tumor size 、histological grade 、family history(P ＜ 0.05).The average survival period of high expression group was significantly shorter than that in low expression group (P ＜ 0.05).The high expression of RIN1 was related to the tumor disease-free survival (P ＜ 0.05).mRNA and protein were expressed in 20 cases of breast cancer and adjacent tissues.The expression of RIN1 mRNA and its protein significantly increased in cancer tissues.Conclusions RIN1 expression plays a role in breast cancer pathogenesis and Maybe its high expression could be used as a prognostic marker in breast cancer.