Objective To understand the AIDS knowledge, behavior and HIV, syphilis, hepatitis C infection status among MSM (men who have sex with men )population from Yulin City, and provide the basis for developing the intervention measures. Methods The monitoring objects were recruited through the internet chat groups and expanded by the way of "snowball". A face to face questionnaire survey was conducted anonymously after getting consent of investigated objects. The contents of the questionnaire included demographic information, previous HIV antibody test results and behavior information. Each subject was collected 3-5ml of venous blood for detection of HIV, syphilis and HCV antibody. Results Between 2010-2014 we investigated 393 MSM persons, who were mostly 20-29 years old young people, unmarried with relatively high level of education. From 2010 to2014,the annual awareness rate of AIDS knowledge were 98.2%, 94.4%, 98.0%, 96.0% and 98.0% ,respectively; the condom use rate during every the homosexual contact in the last six months were 48.5% , 36.4% , 41.2% , 47.3% and 90.3% , respectively; the annual positive rate of HIV were 1.8%, 5.6%, 5.1%, 3.0% and 12.9%, respectively; the positive rate of syphilis were 2.8%, 0.0% . 8.9%, 2.0% and 0.0%, respectively; and no positive HCV infection was detected. Conclusion Among MSM population from Yulin City the AIDS knowledge awareness rate is high. The rate of condom use increased year by year, but unprotected high risk sexual behavior still exists, resulting in AIDS spreading in the population. For preventing the spread of AIDS in the MSM population propaganda on the harmful results of AIDS and promotion of condom use should be strengthened.%目的：了解玉林市男男性行为者（MSM）的艾滋病知识、行为特征及艾滋病、梅毒和丙肝感染状况和流行因素，为制定玉林市艾滋病防治干预措施提供依据。方法通过网络聊天群及“滚雪球”的方式招募监测对象，由经过培训的工作人员开展面对面问卷调查，每人采集3～5mL静脉血进行艾滋病、梅毒、丙肝抗体检测。结果2010—2014年共调查 MSM 人群393名，以20～29岁的青壮年人群为主，未婚居多，文化程度相对较高；每年调查的艾滋病知识知晓率均在94%以上，最近一次与同性肛交时使用安全套的比例高于未使用安全套的比例，差异有统计学意义（χ2=14.381，P =0.005）。2010—2014年，每年最近六个月与同性肛交时每次都使用安全套的比例分别为48.5%、36.4%、41.2%、47.3%和90.3%；每年 HIV 阳性检出率分别为1.8%、5.6%、5.1%、3.0%和12.9%，梅毒阳性检出率分别为8.9%、2.8%、0.0%、2.0%和0.0%，未检出 HCV 阳性。结论玉林市 MSM 人群艾滋病知识知晓率较高，安全套使用率呈逐年上升，但是无保护高危性行为仍然存在，造成艾滋病在该人群中扩散流行。应加强艾滋病危害性、严重性的宣传教育，积极引导推广使用安全套，进一步遏制艾滋病在 MSM 人群中的扩散蔓延。
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