季节对大叶桉和柠檬桉叶挥发油化学成分的影响及抑菌性研究

摘要

The volatile oils from leaves of Eucalyptus robusta and Eucalyptus citriodora were obtained using hydrodistillation,and the composition was analyzed by GC-MS. The effect of season on the volatile constit-uents and antimicrobial activities was also investigated. The results showed that the contents of volatile oils from both Eucalyptus robusta leaf and Eucalyptus citriodora leaf were higher in spring,highest in winter. The major compositions of the Eucalyptus robusta leaf were 3-carene,α-terpineol,( -)-eucalyptus alco-hol,d-limonene and l-pinocarveol. The major compositions of volatile oil from the Eucalyptus citriodora leaf were citronellal, isopulegol, and β-citronellol. In spring, the content of 3-carene and citronellal reached the highest. Besides,the strong antibacterial effects of the two volatile oils against common patho-genic bacteria were found.%采用水蒸气蒸馏法提取桉叶挥发油，用GC-MS法分析挥发油的主要成分，考察了季节对大叶桉和柠檬桉两种桉叶挥发油化学成分的影响及抑菌活性。结果表明，两种桉叶挥发油含量在春季(1~3月)和冬季(10~12月)较高，其中冬季挥发油含量最高。大叶桉叶挥发油主要成分为3-蒈烯、α-松油醇、(-)-蓝桉醇、D-萜二烯、1-松香芹醇；柠檬桉叶挥发油主要成分为香茅醛、异胡薄荷醇、β-香茅醇。大叶桉挥发油中的代表成分3-蒈烯和柠檬桉挥发油中的代表成分香茅醛在春季(1~3月)含量为最高。两种挥发油对常见致病菌有良好的抑制作用。