柚皮素分子印迹膜的制备及评价

摘要

Chitosan(CS)as a functional polymer,polyvinyl alcohol(PVA)as additive,polyethylene glycol (PEG)as porogen,the aqueous phase blend,cast prepared CS/PVA blend film CS molecularly imprinted membrane as the basic experimental conditions.Basis of molecular imprinted membrane preparation con-ditions and processes was optimized by experimental investigation.The results show that,when mNG∶mCS =1/15,the maximum capacity of imprinted membrane.Compared with non-imprinted membrane,the greater by the amount of NG,NG and the imprintd infiltration capacity is proportional to the amount of non-tem-plate molecule endothelin in the imprinted membrane,and Jue penetration rate is low.In the same concen-tration,when the base film composed of PVA/CS=0.6/1 .4,NG and competition properties of molecular imprinting Jue skin pigment separation factor is 10.09.When the composition of PVA/CS is 1.4/0.6,the separation factor is 8.40.%以壳聚糖（CS）为功能聚合物，聚乙烯醇（PVA）为添加剂，聚乙二醇（PEG）为致孔剂，水相共混，流延法制备了CS／PVA共混膜作为制备CS分子印迹膜的基本实验条件。实验优化了分子印迹膜的基础制备条件和工艺。在选定的膜配比下，以柚皮素（NG）为印迹分子，乙醇为洗脱剂，水相共混包埋制备了以NG为模板分子的壳聚糖分子印迹膜。结果表明，当m（NG）∶m（CS）＝1／15时，印迹膜的容量最大。印迹膜较之非印迹膜，对NG有较大的透过量，NG分子的渗透量与印迹容量成正比，非印迹分子槲皮素在印迹膜上的渗透速率较低。相同浓度下，基膜组成为PVA／CS＝0．6／1．4时印迹分子NG与竞争物桷皮素的分离因子为10．09，组成为PVA／CS＝1．4／0．6时印迹分子NG与竞争物槲皮素的分离因子为8．40。