目的 探讨紫檀芪介导PI3K/Akt信号通路对人骨肉瘤细胞凋亡、迁移能力和活性的影响.方法 收集人骨肉瘤细胞系SOSP-9607.细胞活力检测采用MTT法,细胞周期分析和细胞凋亡分析应用流式细胞仪,采用细胞划痕和黏附检测转染后SOSP-9607细胞的体外迁移能力和黏附能力.结果 MTT检测显示,加入浓度为1、2、4 μmol·L-1的紫檀芪培养后,细胞增长出现抑制,且呈剂量和时间依赖性.与对照组相比,不同药物浓度下骨肉瘤细胞的凋亡指数逐渐增加;细胞发生G0~G1期阻滞.随着药物浓度增加,划痕边缘间距逐渐扩大,细胞黏附性呈显著下降趋势.随着药物浓度增加,Cyclin D1表达下调,Bax和Bak水平升高,而p-JAK2、p-STAT3和Bcl-xL水平下降(P<0.05).结论 紫檀芪具有很强的抗人骨肉瘤细胞活性的作用,可降低骨肉瘤细胞的黏附能力及迁移能力,通过介导JAK2/STAT3信号通路导致细胞周期阻滞,抑制细胞生长,诱导细胞凋亡.%Objective To investigate the effects of pterostilbene on apoptosis, migration ability and activity of human osteosarcoma cells by mediating PI3K/Akt signaling pathway. Methods Human osteosarcoma cell line SOSP-9607 was selected as the subject. Cell viability was examined by MTT; cell cycle analysis and apoptosis analysis were detected by flow cytometry; cell scratch, and adhesion assays were used to detect the SOSP-9607 cell migration in vitro and adhesion ability. Results MTT assay showed that the growth of cells was inhibited by adding 1, 2 and 4 μM pterostilbene after the culture in dose and time dependent manner; Compared with the control group, cell adhesion was decreased. At the same time, the apoptotic index of osteosarcoma cells increased gradually at different concentrations compared with the control group; and cell stage G0~G1was blocked. As the increase of concentration, the edge distance of scratches gradually enlarged. At the same time, cell adhesion showed a significant downward trend with the increase of concentration. With the increase of the treatment con-centration, the expression of Cyclin and D1 decreased, the levels of p-JAK2 and p-STAT3 decreased, the levels of Bax and Bak increased, while the levels of Bcl-xL decreased (P< 0.05). Conclusion Pterostilbene has a strong anti-human osteosarcoma cell activity, and can re-duce the adhesion and migration ability of osteosarcoma cells. By mediating the JAK2/STAT3 signaling pathway, it leads to cell phase arrest of G0~G1and cell growth inhibition, and induce apoptosis of cells.
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