液相色谱-串联质谱法研究地克珠利在家兔组织中残留及消除规律

摘要

[目的]建立一种检测家兔组织中地克珠利含量的液相色谱-串联质谱法（LC—MS／MS），研究地克珠利在家兔组织中的残留及消除规律。[方法]选择48只家兔（雌雄各半），在饲料中加入10g／1000kg地克珠利混饲，连续饲喂30d。分别于休药后的第0、1、2、3、4、5、6天，每个时间点随机处死6只家兔（雌雄各半），取肌肉、肝脏、肾脏组织，DMF，乙腈沉淀法提取并用LC—MS，MS法检测地克珠利残留量。[结果]地克珠利在肾脏中的残留量最高（休药0d为912．6μg／kg），肝脏次之（休药0d为156．μg／kg），肌肉最低（休药0d为43．1μg／kg），在肾脏中的消除速度最快。[结论]该方法适于检测家免组织中的地克珠利含量。休药0d即可满足欧盟要求%[Objective ] The research aimed to set up a method of LC-MS/MS for detecting the residues of dielazuril in rabbit tissues and investigate the residue and elimination laws of diclazuril in rabbit tissues. [ Method ] Forty-eight rabbits （24 male and 24 female）were fed with the diet that contained 10 g diclazuril per 1 000 kg diet for 30 days. Six rabbits （3 male and 3 female）were killed at eacil time point （ 0, 1,2,3,4,5, 6 days after the last drug administration）. The muscle,liver and kidney tissues of rabbits were picked to extract by DMF/acetonitrile precipitation method. The residual amount of diclazuril in these tissues was determined by LC-MS/MS. [ Result ] The residual amount of diclazuril in kidney of rabbits was the highest and that on the day of the last drug administration was 912.6 μg/kg. The residual amount of diclazuril in liver of rabbits was the second and that on the day of the last drug administration was 156.1 μg/kg. And the residual amount of diclazuril in the muscle of rabbits was the lowest and that on the day of the last drug administration was 43.1 μg/kg. The elimination rate of diclazuril in the kidney was the fastest. [Conclusion] The method was suitable for the determination of diclazuril residues in rabbit tissues. The residual amount of diclazuril on the day of the last drug administration could meet the standards of European Union.