奶牛子宫内膜组织体外培养方法的建立及前列腺素E2和F2α受体分布的研究

摘要

Objective] To establish the cultivation method of bovine endometrial tissue in vitro and to detect the expression of the receptors of PGE2 and PGF2αin the cultured endometrium. [Methods] The fresh endometrial tissue samples from bilateral uterine horn of health dairy cows in proestrus were collected and used for suspension cultivation in vitro. The cultured endometrium was histologically identified, and the expression of the receptors of PGE2, including EP1, EP2, EP3(EP3A、EP3B、EP3C、EP3D), EP4, and the receptors of PGE2αFP were evaluated by RT-qPCR. [Results] The histological assessment indicated that the structure of cultured bovine endometrium in vitro was intact, and the morphology of the glands and nuclear of the cells was normal. The expression of all the tested receptors of PGE2 and PGF2αwere found in the cultured samples from bilateral uterine horn of dairy cows in proestrus. The expression of the receptors varied, the highest relative expression was observed in EP4, and the expression of EP1, EP3B and FP was relatively lower. [Conclusion] The in vitro cultivation method of bovine endometrial tissue was successfully established. The expression of the receptors of PGE2 and PGF2αwere found in cultured endometrium, and the relative expression of different types of receptors varied.%[目的]建立体外培养荷斯坦奶牛子宫内膜组织的方法及检测子宫内膜组织中前列腺素E2和前列腺素F2α受体的分布情况。[方法]挑选新鲜、健康的发情前期奶牛子宫内膜组织，采集双侧子宫角部位内膜组织，体外悬浮培养组织小块并通过组织学形态鉴定培养效果。通过RT-qPCR方法检测PGE2受体EP1、EP2、EP3（EP3A、EP3B、EP3C、EP3D）、EP4和PGF2α受体FP的表达情况。[结果]HE染色表明，体外培养的奶牛子宫内膜组织结构完整，细胞和腺体形态完好，细胞核完好；发情前期奶牛子宫角部位内膜组织中PGE2和PGF2α受体均有表达，且表达量存在差异，其中PGE2受体EP4相对表达量最高，而EP1、EP3B及PGF2α受体FP的相对表达量较低。[结论]成功地建立了奶牛子宫内膜组织的体外培养方法；PGE2和PGF2α的受体在奶牛子宫内膜组织中均有表达，但不同受体的表达量有所不同。