杨树芽提取物清除DPPH自由基的作用

摘要

目的 对杨树芽进行初步提取分离,测定其总黄酮含量和抗氧化活性.方法 通过超声辅助提取杨树芽中有效成分,以抗坏血酸、生育酚和芦丁为对照品,采用DPPH法测定其自由基清除作用.结果 超声辅助提取的杨树芽提取物中总黄酮含量为14.4%,浓度为0.05 g·L-1时,清除自由基能力大于芦丁和生育酚,浓度提高后略低于生育酚,高于芦丁,且清除能力随浓度提高而提高.结论 杨树芽提取物中总黄酮含量较高,并有较强的抗氧化活性.%Objective To make preliminary extraction and separation of poplar buds , and to determination its total flavonoids and antioxi -dant activity. Methods By ultrasound we extracted the active ingredients in the poplar buds . Ascorbic acid,tocopherol and rutin as reference, we measured free radical scavenging effect by DPPH method . Results Ultrasonic assisted extraction of poplar buds contained 14. 4% of total flavonoids, whose radical scavenging capacity was higher than rutin and tocopherol when its concentration reached 0.05 g·L . With increased concentration the scavenging capacity was slightly lower than tocopherol but higher than rutin . And the capacity increased with the increase of concentration . Conclusion Poplar bud extracts had high flavonoid content and strong antioxidant activity .