Objective To establish an HPLC method for determination of vitamin E in compound angelicae dahuricae radix cream. Methods Waters e2695 HPLC included empower waters software,waters 2489 UV detector. And platisil ODS column( 150 mm ×4. 6 mm,5 μm )was used. Mobile phase was methanol,the flow rate was 1.0 mL · min-1 and the detection wavelength was 284 nm. The column temperature was set at room temperature. Injection volume was 20 μL. Results The linear range of vitamin E was 82. 76 ～ 1 655. 2 mg · L-1( r =0.999 9 ). The average recovery was 100. 5%( n =9 ),and RSD was 0. 87% . Conclusion This method was simple,sensitive, and accurate for the quality control of compound angelicae dahuricae radix cream.%目的 建立高效液相色谱法测定白芷维生素E乳膏中维生素E 的含量.方法 Waters e2695 HPLC色谱系统;Empower Waters 工作站;Waters 2489 UV检测器;Platisil ODS(150 mm×4.6 mm,5 μm)色谱柱;流速:1.0 mL·min-1 ;流动相:甲醇;检测波长284 nm,进样量为20 μL.结果 维生素E在82.76～1 655.2 mg·L-1 的范围内呈良好的线性关系(r=0.999 9).维生素E的平均加样回收率为100.5%(n=9),RSD=0.87%.结论 该方法操作简便、准确、稳定、灵敏度高,适用于白芷维生素E乳膏中维生素E的质量控制.
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