碳青酶烯类抗生素耐药铜绿假单胞菌外排泵机制的初步研究

摘要

Objective To study the expression of effiux pumps in carbpenem - resistant Pseudomonas aeruginosa, and to lay a foundation for further study on the mechanism of multidrug - resistant in Pseudomonas aeruginosa. Methods The minimum inhibitory concentrations (MIC) of Imipenem and Meropenem with or without MC207110 were determined by agar dilution method. Results The optimum concentration of MC207110 is 20pg/ml. In 198 isolates multidrug - resistant Pseudomonas aeruginosa, inhibitor rates of Imipenem and Meropenem are 55.49％ and 67.15％, respectively. Conclusion Efflux pump are the main resistant mechanism of Pseudomonas aeruginosa to Meropenem and Imipenem. And MC207110 has the potentiality as a tool for studying the efflux pump mechanism of resistant bacteria.%目的 研究外排泵在碳青酶烯类抗生素耐药铜绿假单胞菌中的表达情况,为进一步研究其多蘑耐药机制奠定基础.方法 采用M-H琼脂稀释法,测定添加和不添加抑制剂MC207110时铜绿假单胞菌对亚胺培南和美罗培南的MIC值.结果 MC207110最佳使用浓度为20 μg/ml.198株多重耐药铜绿假单胞菌对亚胺培南和美罗堵南的MIC值下降4倍以上的菌株分别有55.49%和67.15%.结论 外排泵机制是导致美罗培南和亚胺培南对铜绿假单胞菌耐药的主要机制之一.MC207110可以作为研究细萧外排泵机制的一种较好抑制剂.