孕早期HCMV感染对子代大鼠空间学习记忆功能的实验研究

摘要

目的 探讨孕早期人巨细胞病毒(HCMV)感染对子代大鼠空间学习记忆功能的影响.方法 建立HCMV 先天性感染的大鼠模型,从实验组和对照组各随机抽取6只3 月龄的子代大鼠,采用Morris 水迷宫和跳台实验评价子代大鼠的空间学习记忆功能.结果 水迷宫实验结果示实验组大鼠到达平台的潜伏期和总路程较对照组明显延长(P <0.05),空间探索实验结果也显示:实验组与正常组比较差异有统计学意义(P <0.05).跳台实验中实验组潜伏期明显缩短(P <0.05),跳台实验错误次数也明显增加(P <0.05).结论 孕早期HCMV 感染导致子代大鼠空间学习记忆能力受损.%Objective To explore the effect of HCMV congenital infection on spatial learning and memory function of offspring rats. Methods Rats model of HCMV congenital infection was established. Six 3—month—old offspring rats were randomly selected from experimental group and control group, using the Morris water maze and platform experiment to evaluate their spatial learning and memory function. Results Morris water maze test results showed the latency and total distance of experimental rats to reach the platform were significantly longer than those of the control group (P<0.05); space exploration experiment results showed that experimental group and normal group had significant difference (P<0.05); platform experiment showed latency of experimental group was significantly shorter (P<0.05), and error frequency in step—down test also significantly increased(P<0.05). Conclusion HCMV congenital infection can injure offspring rats in spatial learning and memory function.