3种牙髓生物活性材料对小鼠间充质干细胞骨向分化的影响

摘要

Objective To assess the biocompatibility of three kinds of endodontic bioactive materials, i. e. , mineral trioxide aggre-gate ( MTA) , bioaggrregate ( BA) and biodentine ( BD) , and to investigate the effects of these cements on osteogenic differentiation of the Mus musculus mesenchymal stem cells ( MSCs) . Methods The cell viability, mineralization and differentiation ability of Mus musculus MSCs were evaluated by XTT assay and ALP staining, and the effects of MTA, BA and BD on osteogenic differentiation of the MSCs were ob-served respectively. Results The cell viability of MSCs in various concentrations of BD (1, 1/2 and 1/4) were all significantly lower than that of MTA and BA (P<0. 001), but there was no significant difference in the cell viability among the three cements when their concentra-tions dropped to 1/10 and 1/50. In the ALP staining indicating mineralization and differentiation ability of MSCs, MTA, BA and low concen-tration of BD all led to an increase in the tinctural value when compared with the controls (P<0. 05). Conclusion MTA, BA and low con-centration of BD have good biocompatibility with the Mus musculus MSCs. Meanwhile, they have motive effects of mineralization and differen-tiation during the course of osteogenic differentiation of the murine MSCs, suggesting that these cements may be used as the root-end filling material of root canal.%目的：评估3种牙髓生物活性材料———三氧化聚合物( MTA)、Bioaggregate( BA)和Biodentine( BD)的生物相容性并观察其各自对小鼠间充质干细胞( MSCs)向成骨分化的影响。方法采用XTT实验和ALP染色检测小鼠MSCs生存能力、分化矿化能力,观察MTA、BA及BD对小鼠MSCs向成骨分化的影响。结果 BD的细胞生存能力在浓度1、1/2、1/4时明显低于MTA和BA(P<0．001),3种材料的细胞生存能力在材料浓度降至1/10和1/50时,差异无统计学意义(P>0．05)。 MTA、BA和低浓度BD在显示分化矿化能力的ALP染色检测方面,与对照组相比染色值均升高,差异有统计学意义(P<0．05)。结论 MTA、BA以及低浓度BD与小鼠MSCs有良好的生物相容性；MTA、BA和低浓度BD在小鼠MSCs向成骨方向分化过程中有促进分化矿化作用,可以作为根管的根尖封闭材料。