A method for determination of odorants in human blood had been developed with Gas Chro-matography-Mass Spectrometry (GC-MS) combined with Head Space Solid Phase Micro-Extraction (HS-SPME), and the extraction conditions of SPME were optimized. Nine compounds in human bloods were I-dentified and then used to compare the blood odor profiles. These compounds were allyl methyl sulfide, cyclohexanol, 2-ethyl hexanoic acid, 3,7-dimethyl-6-octen-l-ol, 2-n-octyl-cyclepentene, 2-methyl-3-decen-5-one, ethyl 2-hexenoate, 2-methyl butanal, tributyl phosphate. The results demonstrated that there was difference in blood odors between humans and animals. The origins of bloods might be determined based on their odor profiles.%建立了顶空固相微萃取-气相色谱质谱联用检测血液气味的方法,优化了固相微萃取条件,对人、鸡和犬血液气味的差异性进行了比较研究.以人体血液气味中存在的甲基烯丙基硫醚、2-乙基己酸、香茅醇、3-辛基环戊烯、2-甲基-3-葵烯-5-酮、2-己烯酸乙酯和磷酸三丁酯等9种化合物作为目标化合物进行比较分析.结果表明,人和动物血液气味的化学组成存在显著差异性,利用血液气味的差异性能够区分不同来源的血迹.
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