丛枝菌根对黄檗氮素及含氮物质含量的影响

摘要

[目的]研究丛枝菌根对黄檗氮素及含氮物质含量的影响。[方法]通过盆栽试验，用丛枝菌根真菌接种黄檗一年生实生苗，研究丛枝菌根对黄檗（Phellodendron amurense Rupr.）氮素及含氮物质含量的影响。[结果]黄檗幼苗形成丛枝菌根后，增加了叶片氮素含量，接种G．mosseae的苗木叶片氮素含量比对照提高了1．28～1．60倍，光合能力增强。接种丛枝菌根菌后，显著提高了黄檗叶绿素含量及叶绿素a和b的比值，增强了黄檗光合作用的能力，叶绿素a含量提高了25％以上；同时，增加了植物体内吲哚乙酸的含量，增加比例在1．65～2．41倍之间；增强植物体内硝酸还原酶的活性，增加了可溶性蛋白含量，增加比例在1．67～2．49倍之间，增强了植物氮素代谢能力，促进植物生长，增强植物的次生代谢能力。[结论]该研究结果为丛枝菌根真菌在黄檗上的应用提供了理论依据。%[Objective] This study aimed to explore the effect of arbuscular mycorrhiza on the content of nitrogen and nitrogenous matter in amur corktree（Phellodendron amurense Rupr.）seedlings. [Method] The annual seedlings of Phellodendron amurense Rupr. were inoculated with four arbuscular mycorrhiza fungi in a pot experiment to study the influences of arbuscular mycorrhiza on the content of nitrogen and nitrogenous matter in Phellodendron amurense Rupr. [Result] After inoculation with arbuscular mycorrhiza fungi, the Phellodendron amurense Rupr. seedlings developed arbuscular mycorrhiza, leading to an enhancement of photosynthetic capacity. The leaf nitrogen content of those inoculated with Glomus mosseae increased to 1.28- 1.60 times as compared with the control. The chlorophyll content and chlorophyll a/b ratio were also raised, with an increase over 25% of chlorophyll a content. In addition, IAA content in plants increased to 1.65-2.41 times; and nitrate reductase activity was also enhanced, as well as soluble protein content, 1.67-2.49 times as high as the control, which improved the nitrogen metabolic ability, and promoted the plant growth, as well as the secondary metabolic ability. [Conclusion] This study provides a theoretical basis for the application of arbuscular mycorrhiza on Phellodendron amurense Rupr.