邻苯二甲酸酯降解菌的分离鉴定及降解特性

摘要

利用平板分离技术，以5种邻苯二甲酸酯类物质（DMP、DEP、DBP、DEHP、DOP）为能源和碳源，对巢湖底泥进行驯化培养，从中筛选出活性菌株 DM1，经鉴定，该菌为皮氏伯克霍尔德氏菌（Burkholderia pickettii）。气相色谱分析的结果表明：B. pickettii.z-1菌对五种混合体系邻苯二甲酸酯的降解趋势符合一级动力学方程：，且随着基质邻苯二甲酸酯浓度梯度的增加，PAEs的降解速率减小。B. pickettii.z-1菌对不同PAEs化合物的降解速率差别很大，较短侧链的 DMP和 DEP降解较快，较长侧链的DEHP、DOP降解较慢。%By using plate screening techniques with five phthalate esters (DMP, DEP, DBP, DEHP and DOP) as energy and carbon sources, an active strain was isolated from the sediments of Chaohu Lake, which was identified as Burkholderia picketti and named B. picketti .z-1. The biodegradation of five phthalate esters by B. pick-etti .z-1 strain was in accordance with the pseudo first-order kinetic equation: Ct=C0·e-kt. As the concentration of phthalate esters increased, the degradation rate of phthalate esters was reduced. B. picketti .z-1 strain exhibited remarkably different degradation effects on various PAEs. Specifical y, short-side-chain DMP and DEP were degraded rapidly, while long-side-chain DBP and DEHP were degraded slowly.