“慢呼宁”口服液对鸡安全性的试验研究

摘要

为了在对“慢呼宁”口服液的安全性进行评价,为临床用药提供安全依据,选取120羽14日龄的三黄肉鸡,适应性饲喂1周后,随机分为治疗剂量的1倍剂量组、3倍剂量组、5倍剂量组和对照组,每组30羽.试验时分别按照每组试验鸡的平均体质量给予不同剂量的药物,连续给药1周.在试验前和试验结束时,各组随机选取10只进行称质景和采血,测定体质量变化、血常规和肝肾功能指标,测定脏器指数,并对鸡的心、肝、脾、肺和肾脏进行病理检查.结果表明,用药后各组鸡的体质量增加量及脏器指数组间差异不显著(P＞0.05).血常规指标则在试验前后组间差异亦不显著(P＞0.05);血清ALT活性、白蛋白、肌酐和尿素含量组间差异不显著(P＞0.05);在用药后,各组鸡的心、肝、脾、肺和肾脏组织变化均无显著差异和异常.结果表明,“慢呼宁”口服液对鸡生理生化指标的影响小,病理剖检未见对心、肝、脾、肺和肾脏产生毒性作用,是一种低毒安全的中兽药制剂.%This study aimed to evaluate the safety of Manhuning oral liquid and to provide safety basis for clinical treatment.A total of 120 chickens of 14 days old were selected.After one week of adaptive feeding,they were evenly and randomly divided into four groups:one time the therapeutic dose (T1),three times the therapeutic dose (T2),five times the therapeutic dose (T3) and control (CK).In different groups,the chickens were fed with Manhuning oral liquid according to the set doses for one week.At the beginning and at the end of the experiment,total 10 chickens were selected randomly from each group,respectively.They were weighed,and their blood was sampled for determination of routine blood indexes and examination of liver and kidney function.Organ indexes were determined,and pathological examination was conducted for the heart,liver,spleen,lung and kidney of the chickens.The results showed that the body mass gain and organ indexes differed insignificantly among different groups (P＞0.05).No significant differences were found in the routine blood indexes,serum ALT activity,albumin content,creatinine content and urea content among different groups before and after the experiment (P＞0.05).After the administration,the heart,liver,spleen,lung and kidney of the chickens showed no obvious abnormalities.In short,Manhuning oral liquid had little impact on the physiological and biochemical indexes and had no toxic effects on the heart,liver,spleen,lung and kidney of chickens,and it is a low-toxicity safe veterinary drug preparation.