乳酸菌复合系SFC-2增菌培养基的优化

摘要

通过对乳酸菌复合系SFC-2培养基的组分进行响应面优化,从而得出SFC-2优化培养基配方为白砂糖10 g/L、蛋白胨31.36 g/L、牛肉膏15.57 g/L、酵母膏10 g/L、乙酸钠7.3 g/L、柠檬酸三铵4 g/L、K2HPO44 g/L、MgSO4·7H2O0.58g/L、MnSO4·H2O0.25 g/L.SFC-2在此培养基中经30℃,16h培养,其活菌数可达到4.0×108 cfu/mL,较MRS-S中(8.2× 107 cfu/mL)提高近5倍.