在线固相萃取－HPLC 法测定公共绿地土壤中新烟碱类农药残留

摘要

The target compounds in soil samples , including: nitenpyram , thiamethoxam , imidacloprid , acetamiprid and thiacloprid , were extracted by the mixed solution of acetonitrile and dichloromethane under ultrasonic operation , and then purified with a PEP on-line SPE column .Analytes were determined by high performance liquid chromatography method .By optimizing the test conditions , good linear responses of five neonicotinoid residues were observed in the range of 0.01 mg/L~2.00 mg/L.Method detection limits of these five neonicotinoid pesticides in soil ranged from 0.002 mg/kg ~0.003 mg/kg.The recoveries were 72.5%~119%,while RSDs were all below 9 .6%.%土壤样品经二氯甲烷－乙腈混合溶液超声提取后，用PEP固相萃取柱在线净化，再用HPLC法测定样品中吡虫啉、啶虫脒、噻虫嗪、噻虫啉、烯啶虫胺等5种新烟碱类农药残留。通过优化试验条件，使该方法在0．01 mg／L～2．00 mg／L范围内线性良好，当取样量为3．00 g时，方法检出限为0．002 mg／kg ～0．003 mg／kg。8种土壤样品3个质量比水平的加标回收率为72．5％～119％，RSD均低于9．6％。