温针灸减低阿司匹林抗风湿致胃肠道副作用的研究

摘要

Objective To observe the effect of warm-needle moxibustion on the gastric mucosal injury caused by antirheumatic dose of Aspirin.Methods Forty SD rats were equally randomized into blank group, model group, warm needling group and Omeprazole (for relieving peptic ulcer) group, with 10 rats in each.The rats were forced to undergo 12 hours' fasting and 4 hours' water-deprivation before the experiments.Normal saline (10 mL/kg) was given to the rats in the blank group.Gastric mucosa lesion model was established by intragastric infusion of aspirin (420 mg/kg).Warm needling was applied to "Zhongwan" (CV 12), "Xiawan" (CV 10), "Zusanli" (ST 36) and "Neiguan" (PC 6) for 30 min, once a day for 7 days.The rats of the Omeprazole group were fed with Omeprazole( 10 mg/100 g body weight) once a day for 7 days.According to Guth's method, the ulcer index (UI) of the gastric mucosa was calculated following killing the rats.The activity of superoxide dismutase (SOD) and the levels of malondialdehyde (MDA) in the serum and gastric mucosa were detected with hydroxylamine method and thiobarbituric acid method, respectively.Results Compared with the blank group, the gastric UI, MDA levels of both serum and gastric mucosa in the model group were increased obviously (P＜0.01), while serum and gastric mucosal SOD activity in the model group were decreased considerably (P＜0.01 ).In comparison with the model group, the gastric UI, and both serum and gastric mucosal MDA contents in the warm needling group and Omeprazole group were down-regulated significantly (P＜0.01), whereas both serum and mucosal SOD activity in the warm needling group and serum SOD activity in the Omeprazole group were up-regulated significantly (P＜0.01).Comparison between the warm needling group and Omeprazole group showed that the UI, both serum and gastric mucosal SOD activity, and serum MDA level of the former group were evidently higher than those of the Omeprazole group (P＜0.05), but gastric mucosal MDA level of the warm needling group was apparently lower than that of the Omeprazole group (P＜0.05).Conclusion Warm-needle moxibustion can reduce the gastric lesion (ulcer) caused by aspirin in the rat,which may be closed associated with its effects in up-regulating both serum and gastric mucosal SOD activity and down-regulating both serum and gastric mucosal MDA levels.%目的:探讨温针灸减低阿司匹林致胃肠道副作用的疗效及机制.方法:用随机数字表法将SD大鼠分为空白组、模型组、温针灸组与奥美拉唑组,每组10只.阿司匹林灌胃造模.灌胃后温针灸组取"中院""下脘"、双侧"足三里"与双侧"内关",平补平泻手法针刺穴位后用温针灸架进行温针灸30min,每日1次,连续治疗7d.参照Guth标准测量大鼠胃溃疡指数,黄嘌吟氧化酶法(羟氨法)测血清和胃钻膜的超氧化物歧化酶(SOD)活力,硫代巴比妥酸法测其丙二醛(MDA)含量.结果:与空白组比较,模型组各指标均有明显变化(尸<0.01);温针灸组的血清和胃私膜SOD活力均提高,MDA含量均降低,清疡指数减小,与模型组比较差异有统计学意义(P<0.01);奥美拉吐组的溃疡抑制率优于温针灸组(P<0.05),但是温针灸组在提高血清和胃钻膜SOD活力和降低胃私膜MDA含量方面优于奥美拉唑组(P<0.05).结论:温针灸能明显减低阿司匹林所致的胃肠道副作用,其机制可能与杭氧自由基损伤有关.