火针对类风湿性关节炎大鼠血清白介素-1和肿瘤坏死因子-α的影响

摘要

目的:观察火针对类风湿性关节炎大鼠血清白介素-1(IL-1)和肿瘤坏死因子-α(TNF-α)的影响,探讨火针治疗类风湿性关节炎的作用机制.方法:将40只雌性Wistar大鼠随机分为正常组、模型组、甲氨蝶呤(MTX)组、火针组.于大鼠右后足跖底部皮内注射0.1mL完全弗氏佐剂建立类风湿性关节炎模型.火针点刺“夹脊穴”“足三里”和“阿是穴”,共治疗8次.容积法测量大鼠右后足肿胀度；双抗体夹心ABC-ELISA法检测大鼠血清IL-1和TNF-α含量；光镜观察大鼠膝关节滑膜组织的病理变化.结果:模型组大鼠足部肿胀度、血清IL-1和TNF-α的含量较正常组显著升高(P＜0.01)；MTX组造模后33 d足部肿胀度较模型组降低(P＜0.01),火针组造模后19、26、33d足部肿胀度较模型组降低(P＜0.01),治疗后MTX组和火针组大鼠血清IL-1和TNF-α的含量较模型组均明显降低(P＜0.05,P＜0.01),火针组与MTX组相比大鼠足部肿胀度、血清IL-1和TNF-α的含量明显降低(P＜0.05,P＜0.01).与正常组比较,模型组大鼠滑膜组织有明显的病理改变,各治疗组均能改善膝关节滑膜组织的炎性病变,且火针对滑膜病变的改善优于MTX.结论:火针可降低类风湿性关节炎大鼠血清IL-1和TNF-α的含量,减轻关节局部肿胀度,改善滑膜病变,有效控制类风湿性关节炎的病情发展.%Objective To observe the effect of fire-needle intervention on serum IL-1 and TNF-α contents In rheumatoid arthritis (RA) rats so as to study its underlying mechanism in improving RA. Methods A total of 40 Wistar rats were randomized into normal control, model, methotrexate (MTX, medication) group and fire-needle groups, with 10 cases in each group. RA model was established by injecting Freund's complete adjuvant (0. 1 mL) into the rats' right hind paw. Fire-needle was applied to "Jiaji"(EX-B 2),"Zusanli"(ST 36) and "Ashi point" (the most severe site of the swelling joint), once every three days and 8 times altogether. The rat's right hindpaw swelling volume was detected by volumetric method, and serum IL-1 and TNF-a contents were determined by double antibody sandwich ELISA. The pathological changes of synovium tissue of the right knee-joint were observed by optical microscope. Results In comparison with the control group, the swollen paw volumes at time-points of 12 d, 19 d, 26 d and 33 d after modeling and serum IL-1 and TNF-α contents were increased significantly (P<0.01). Compared to the model group, the swollen paw volumes on day 33 in both medication and fire-needle groups were decreased considerably (P<0.01), while serum IL-1 and TNF-a contents in the medication and fire-needle groups were reduced significantly (P<0. 05. P<0. 01). No significant differences were found between the medication and fire-needle groups In the swollen paw volumes on day 12 (P>0. 05). The pathological states (synovial cells' arrangement, inflammatory infiltration, synovial membrane thickening, etc.) of synovium tissue of the right knee-joint in both medication and fire-needle groups were relatively lighter than those of the model group Conclusion Fire-needle therapy can down-regulate serum IL-1 and TNF-α contents in rheumatoid arthritis rats, and reduce the degree of arthroncus of the affected joint, which may contribute to its effect in improving synovial lesions.