电针对非酒精性脂肪肝大鼠血清视黄醇结合蛋白4及肝功能的影响

摘要

目的:观察针刺对非酒精性脂肪肝(NAFLD)大鼠肝功能指标及视黄醇结合蛋白4(RBP 4),探讨针刺对NAFLD大鼠肝功能及脂肪细胞因子影响的作用机制.方法:将44只SD大鼠随机分为空白组、模型组、电针组和非穴组,每组各11只.喂养高糖高脂饲料制备NAFLD模型.电针组针刺“丰隆”“足三里”“太冲”“三阴交”.用比色法测定血清谷草转氨酶(AST)、丙氨酸转氨酶(ALT)、总胆固醇(TC)、甘油三酯(TG),用酶联免疫法检测血清RBP 4含量.结果:电针组与模型组比较,TC、TG、AST、ALT和RBP4都显著下降(P＜0.05),肝组织内脂肪性变减轻；非穴组与模型组比较血清各指标变化不明显(P＞0.05),肝组织内脂肪性变无明显改善.结论:电针穴位对NAFLD大鼠有较好的治疗作用,其作用机制可能是通过降低血清RBP 4,减轻肝脏负荷,从而改善肝脏功能实现的.%Objective To observe the influence of electroacupuncture (EA) intervention on liver function and retinol binding protein 4 (RBP4) in nonalcoholic fatty liver disease (NAFLD) rats, so as to study its mechanisms underlying improving liver function. Methods A total of 44 SD rats were randomly allocated to normal control, model, EA and non-acupoint groups, with 11 rats in each group. NAFLD model was established by feeding the rats with high glucose and high fat forage for 5 weeks. EA (2 Hz, 1.5V, 15 min) was applied to unilateral "Fenglong" (ST40), "Zusanli" (ST36), "Sanyinjiao" (SP 6) and "Tai-chong" 0. 05). Conclusion EA intervention can lower serum AST, ALT, TC, TS and RBP4 contents in NAFLD rats, which may be responsible for its action in improving NAFLD in clinic.