To investigate the mechanism of TGDGBX regulating expression of collagen in human fetal lung fibroblast ( HLF- I ) triggered by transforming growth factor-β1 ( TGF-β1 ). Methods Fibroblasts was cultured by α-MEM medium and was grouped according to treatment. Groups as follows, A: control ; B: TGF-β1 ( 1 ng/ml ); C: TGF-β1( 1 ng/ml ) + TGDGBX ( 0. 03 mg/ml ); D: TGF-β1(1 ng/ml ) + TGDGBX( 0. 09 mg/ ml ),E:TGF-β1( 1 ng/ml ) + TGDGBX ( 0. 27 mg/ml ). Proliferation of cells was measured by MTT assay. The changes of collagen- I ( COL- I ), collagen - Ⅲ ( COL- Ⅲ ), α-SMA and MMP-9 mRNA was detected by reverse transcription-polymerase chain reaction( RT-PCR ) and the expression of MMP-9 in the supernatants were detected with ELISA. Results The cell multiplication of groupe TGDGBX was significantly higher than that in group TGF-β1( P <0. 05 ); The collagen I ( COL- I ),collagen Ⅲ ( COL-Ⅲ) and a-SMA mRNA expression of fibroblasts in group TGDGBX were significantly lower than that in group TGF-β1( P <0. 05 ),but the expression of MMP-9 mRNA in group TGDGBX was higher than that in group TGF-β1 ( P < 0. 01 ); TGDGBX could induce up-regulation of MMP-9 content of the supernatant. Conclusion TGDGBX can inhibite collagen expression by reducing HFL-I to MFB transdifferentiation and increase the expression of MMP-9 mRNA of HLF- I .%目的 研究当归补血总苷(TGDGBX)对转化生长因子β1(TGF-β1)诱导的人胚肺成纤维细胞(HLF-Ⅰ)转分化及胶原表达的调控作用.方法 体外培养HLF-Ⅰ,根据不同的处理方法分为5组,A组:对照组;B组:加入TGF-β1(1 ng/ml);C组:加入TGF-β1(1 ng/ml)+TGDGBX(0.03 mg/ml);D组:加入TGF-β1(1 ng/ml)+TGDGBX(0.09 mg/ml);E组:加入TGF-β1(1 ng/ml)+TGDGBX(0.27 mg/ml).MMT法检测各组HLF-Ⅰ的增殖情况,羟脯氨酸消化法检测细胞上清液中羟脯氨酸(HYP)含量,ELISA法检测细胞上清液基质金属蛋白酶-9(MMP-9)的含量.半定量RT-PCR法检测Ⅰ型胶原(COL-Ⅰ)mRNA、Ⅲ胶原(COL-Ⅲ)mRNA、α-平滑肌肌动蛋白(α-SMA)mRNA以及MMP-9 mRNA的表达情况.结果 与B组相比,C、D、E组明显抑制TGF-β1诱导的HLF-Ⅰ细胞增殖(P<0.05);降低细胞上清液中HYP的含量(P<0.05);减少HLF-Ⅰ的COL-Ⅰ mRNA、COL-Ⅲ mRNA以及α-SMA mRNA的表达(P<0.05);增加细胞上清液中MMP-9的含量(P<0.05).D和E组与B组相比,显著提高HLF-Ⅰ的MMP-9 mRNA表达水平(P<0.05,P<0.01).结论 TGDGBX可抑制TGF-β1诱导的HLF-Ⅰ转分化和胶原表达,其抑制胶原表达的作用可能是通过增加MMP-9的表达来实现的.
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