Hsf1-潜在的睾丸支持细胞雄激素受体靶基因

摘要

目的 探讨雄激素受体(AR)对睾丸支持细胞中热休克转录因子1(Hsf1)基因表达的影响及其分子机制.方法 采用PCR法及Western blot法检测,AR特异性敲除(S-AR-/y)小鼠和野生型(WT)小鼠睾丸组织中Hsf1的表达,观察雄激素对TM4细胞系中Hsf1和热休克蛋白(HSPs)表达的影响.结果 与WT小鼠比较,S-AR-/y小鼠睾丸组织中Hsf1表达水平升高(P<0.05);雄激素显著降低TM4细胞中热休克转录因子-1(HSF1)表达(P<0.05),并且HSF1能够升高热休克蛋白105(HSP105)和HSP60水平.结论 Hsf1可能是睾丸支持细胞中AR调控的靶基因.%Objective To identify the heat shook transcription factor-l( Hsfl ) as a target gene of AR in sertoli cells and its molecular mechanism in male infertility. Methods PCR and Western blot was used to compare the expression level of Hsfl in sertoli cell-selective knockout AR mice ( S-AR-/y ) with wild type mice ( WT ) and TM4 cell line with or without testosterone. Results Hsfl expression level in S-AR-/y mice was significantly increased compared with WT mice. On condition of testosterone, Hsfl expression of TM4 cell line was significantly reduced in both mRNA and protein level. Moreover, our data suggested that HSF1 acted as a positive factor for target gene expression, in particular regulating heat shock protein 105 ( HSP105 ) and HSP60. Conclusion The Hsfl may plays as a novel target gene of AR in mice sertoli cells.