Objective To observe the survivin/caspase-3 gene proteins expression level in nude mouse with color-ectal carcinoma subcutaneous transplanted by sustained-release fluorouracil implantation, and discuss the mechanism of sustained-release fluorouracil. Methods To establish the colorectal carcinoma subcutaneous transplanted model with LoVo cell used BALB/c nude mice, and divided into 4 groups randomly: the control group respectively the tail intravenous injection of PBS( A group ), the tail intravenous injection of 5-FU group( B group ), the intratu-mor injection of 5-FU group( C group ), the implant of sustained-release fluorouracil group( D group ). After the drugs intervening, contrasting the tumor weight and tumor inhibiting rates, the tumor tissues were observed by HE staining and detected by IHC with the survivin/caspase-3. Results Sustained-release fluorouracil can suppress the tumor growth significantly and it also can enhance the expression of caspase-3( D group:72. 88 ±4. 52 vs A group: 6.75 ±1.67 ,P <0. 05 ),reducing the expression of survivin( D group: 17. 00 ±8.26 vs A group:51.50 ±20.09, P <0. 05 ). Conclusion Sustained-release fluorouracil can develop the anticancer function in apoptosis by enhancing the expression of caspase-3 and reducing the expression of surviving. It can kill the tumor efficiently, and it can promise new ideas in clinical practice.%目的 观察氟尿嘧啶缓释剂植入对人结肠癌裸鼠移植瘤模型中生存素(survivin)及半胱氨酸天冬氨酸特异性蛋白酶-3(caspase-3)基因蛋白表达水平的影响,探讨其作用的相关机制.方法 用人结直肠癌LoVo细胞株建立BALB/c裸鼠皮下移植瘤模型,并将其随机分为4组:PBS尾静脉注射组(A组)、5-FU尾静脉注射组(B组)、5-FU瘤内注射组(C组)、氟尿嘧啶缓释剂植入组(D组).用药干预后,记录瘤重,计算抑瘤率,瘤体进行HE染色,免疫组化法检测survivin及caspase-3表达水平.结果 氟尿嘧啶缓释剂能够显著抑制移植瘤的生长,能显著提高caspase-3的表达[D组(72.88±4.52)与A组(6.75±1.67)比较,差异有统计学意义(P<0.05)],减少survivin的表达[D组(17.00±8.26)与A组(51.50±20.09)比较,差异有统计学意义(P<0.05)].结论 氟尿嘧啶缓释剂可能通过下调survivin的表达、上调caspase-3的表达来发挥其在细胞凋亡方面的抗肿瘤作用,其对肿瘤细胞的有效杀伤,可为临床大肠癌的治疗提供新思路.
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