miR-145对大鼠静脉桥血管内膜增生的影响

摘要

目的探讨微RNA-145(miR-145)对自体静脉桥内膜增生的影响。方法18只雄性SD大鼠均分为慢病毒处理组、慢病毒对照组和未处理组。移植前,慢病毒处理组将移植静脉浸入慢病毒介导的miR-145溶液中(病毒滴度为1．0×109 pfu/ml)15 min,慢病毒对照组将移植静脉浸入空载慢病毒溶液(病毒滴度为1．0×109 pfu/ml)浸泡15 min,未处理组在生理盐水中浸泡15 min。术后2周取出移植血管,应用HE染色和Masson染色观察血管内膜的病理学改变,以及荧光定量 RT-PCR 法检测移植血管中单核细胞趋化蛋白-1(MCP-1)、增殖细胞核抗原(PCNA)、α-平滑肌肌动蛋白(α-SMA)及血管内皮生长因子( VEGF)的表达。结果慢病毒处理组移植血管内膜厚度和管腔狭窄度均较慢病毒对照组和未处理组明显减少, MCP-1、PCNA、α-SMA及 VEGF的表达水平明显低于慢病毒对照组和未处理组。结论 miR-145可以有效抑制移植静脉内膜的增生。%Objective To study the function of miR-145 on intimal hyperplasia in autogenous vein grafts rats. Methods Eighteen male SD rats were divided into three groups: lentivirus treatment group, lentivirus control group and untreated group. Every group has six rats. Before transplantation, the graft which was from lentivirus treatment group was treated in a solution of miR-145 lentivirus ( virus titer was 1 × 109 pfu/ml) at 15 min, the graft which was from lentivirus control group was treated in a solution of lentivirus ( virus titer was 1 × 109 pfu/ml) at 15 min, the untreated group was treated in saline at 15 min. The vein grafts were collected two weeks after transplan-ted, pathological changes of intimal hyperplasia were detected by HE and Masson staining. The expression of mono-cyte chemotactic protein-1(MCP-1),proliferating cell nuclear antigen (PCNA),α-smooth muscle actin (α-SMA) and vascular endothelial growth factor ( VEGF) in vein graft were detected by fluorogenetic quantitation RT-PCR. Results The thickness of intimal and the degrees of restenosis of lumen in lentivirus treatment group were signifi-cantly lower than that in lentivirus control group and untreated group, the expression level of MCP-1, PCNA, α -SMA and VEGF was also significantly lower than that in the lentivirus control group and untreated group. Conclu-sion miR-145 can effectively inhibit the proliferation hyperplasia of vein graft.