TGF-β1和 EGFP 体外电转染兔骨髓间充质干细胞的实验研究

摘要

Objective To observe cases of type Ⅱ collagen expression after transforming growth factor-β1 (TGF-β1) infected in rabbit bone mesenchymal stem cells( BMSCs) by electropration. Methods BMSCs from rabbit were isolated and cultured. 14 days after induction, immunohistochemistry and Western blot methods type Ⅱ colla-gen were used to detect the expression of type Ⅱ collagen. Results CD90 was positive and CD31 was negative by flow cytometry;successfully transfected with TGF-β1 to the BMSCs,the strong expression of type Ⅱ collagen in TGF-β1 group cells was shown by Western blot and immunocy-tochemical stain, which was compared with empty plasmid group and the control group,and the difference was statistically significant(P < 0. 05). Conclusion TGF-β1 plasmid by electroporation to rabbit BMSCs can facilitate expression of collagen Ⅱ.%目的：通过电转染介导转化生长因子β1(TGF-β1)转染兔骨髓间充质干细胞(BMSCs),观察Ⅱ型胶原表达的情况。方法用全骨髓细胞贴壁培养法分离、培养兔 BMSCs；诱导14 d 后,免疫组化和 Western blot 法检测Ⅱ型胶原表达。结果 BMSCs CD90表达阳性,CD31表达阴性；成功转染 TGF-β1至 BMSCs；通过免疫组化及 Western blot 法检测TGF-β1组细胞内Ⅱ型胶原有较强的表达,与增强型绿色荧光蛋白(EGFP)组和空白对照组相比差异有统计学意义(P<0．05)。结论电转 TGF-β1质粒至兔 BMSCs,可以促进Ⅱ型胶原表达。