不同调制方法对晒黄烟叶绿素降解和脂氧合酶活性的影响

摘要

Chlorophyll-a and chlorophyll-b content and the dynamic of lipoxygenase activity, which are important to sensory quality of yellow sun-cured tobacco, were studied by using different curing methods. Results indicated that flue-curing measure could increase Lipoxygenase activity and bulking measure could extend effective time of Lipoxygenase. There was a significant negative correlation between lipoxygenase activity and chlorophyll-a, chlorophyll-b content respectively. Degradation rate of chlorophyll reached up to 90%when treated with yellow sun-curing, flue-curing and bulking measures. Bulking measure was the best as chlorophyll content reached 0.05 mg•g-1DW and its degradation rate was 97.73%.%为提高晒黄烟外观质量，以湖南宁乡晒黄烟全晒方法为对照，在全晒过程中分别引入捂晒、烤晒的方法，研究了调制过程中叶绿素a、叶绿素b和脂氧合酶（LOX）活性的变化。结果表明：烤晒可以提高LOX的活性峰值，捂晒可以有效延长LOX作用时间。叶绿素a含量、叶绿素b含量与LOX活性均呈显著负相关关系。三种调制方式叶绿素降解发生的阶段不同，但调制结束时降解率均达到90%以上，相对而言，T1捂晒效果最好，调制结束叶绿素总量为0.05 mg•g-1DW，降解率为97.73%。