Effect of different pre-treating processes on activity of lipoxygenase from tomato fruits

摘要

Lipoxygenase can act on linoleic acid to produce 9(S)-hydroperoxy-10E, 12Z-octa-decadienoic acid. In order to obtain the highest activity of lipoxygenase from tomato fruits, different factors of pre-treating processes was investigated, including the selection of homoge-nization methods of tomato fruits (Freezing-melting, Ultrasonication, Ultrasonication-freezing, Mechanical comminution, Ultrasonication- mechanical comminution), the pH value of the buffer solution, detergents. Results show that Ultrasonication is the best homogenization method, and 0.05-0.1% Triton X -100( V/V) is required to be added to the buffer solution to acquire the highest activity of lipoxygenase, the best pH value is 6.8.