荧光光谱研究多种印染助剂对活性深蓝M-2GE结合丝素肽的影响

摘要

活性深蓝M-2GE与丝素肽结合为作用距离为3.04nm、结合比为1∶1且能猝灭丝素肽荧光的复合物.常见3种表面活性剂(CTAB、十二烷基苯磺酸钠、曲拉通X-100),3种离子液体(溴化1-乙基-2,3-二甲基咪唑、1-乙基-2,3-甲基咪唑双三氟甲磺酰亚胺盐、氯化羟乙基三甲基胺),3种金属离子(Ca2+、Zn2+、Ba2+),3种淀粉(原淀粉、磷酸酯淀粉、氧化淀粉)和7种氨基酸(L-组氨酸、L-天冬氨酸、L-赖氨酸、L-精氨酸、L-甲硫氨基酸、L-白氨酸、L-脯氨酸)分别加入体系后使二者结合常数增大；而L-苯丙氨酸、L-丝氨酸、L-苏氨酸、甘氨酸,3种氧化剂(Φ=30％H2O2、K2S2O8和K2Cr2O7),聚丙烯醛胺和醛酸酯淀粉浆料以及金属离子Cu2+的存在均导致结合常数变小,因此在活性深蓝M-2GE印染过程中应根据工艺要求选择不同印染助剂.%It is important to study the interaction of small molecules with silk peptide.In this paper,the interaction of reactive dark blue M-2GE (RDBMG) with silk peptide was studied by means of multiple spectroscopy.The binding distance and binding ratio were calculated as 3.04 nm and 1∶ 1,respectively.Additionally,the effects of foreign substances in dyeing system such as surfactants,ionic liquids,metal ions,oxidants,textile pulp,and seven kinds of amino acids (histidine,L-aspartic acid,L-lysine,L-arginine,L-methylthio amino acid,L-leucine and L-proline) on SP-RDBMG were also investigated.The results showed that the binding constants increased when Ionic liquids including chloride hydroxyethyl trimethyl amine,bromide 1-ethyl-2,3-dimethyl imidazolium,1-ethyl-2,3-methyl imidazolium bis [trifluoromethyl-sulfonyl] imide,textile pulp (original starch,phosphate starch,oxidized starch),metal ions (Ca2+,Zn2+,Ba2 +),surfactants (CTAB,Triton X-100,sodium dodecyl sulfate) and seven types of amino acids were added into SP-RDBMG.However,the binding constants decreased when polypropylene aldehyde amine,aldehyde ester starch,Cu2+ and oxidants (Φ =30％ H2O2,K2S2O8,K2Cr2O7) were in the presence of SP-RDBMG.