雌激素功能性调节人内皮细胞的生长调节致癌基因α表达

摘要

目的：研究雌激素在体外调节人脐带静脉内皮细胞rn(HUVEC)的生长调节致癌基因α(GROα).方法：rn以体外培养的HUVEC为模型，Northern法检测CXCrn族趋化因子GROα mRNA；ELISA方法检测细胞表面rn的GROα蛋白表达；静态细胞粘附实验测定细胞表rn面的GROα蛋白的生理意义. 结果：17β-雌二醇rn(0.05 μmol/L)明显抑制HUVEC产生GROα mRNArn和蛋白表达水平；而且17β-雌二醇抑制其蛋白表达rn水平显示剂量依赖性关系；雌激素受体α拮抗剂rn他莫昔芬(0.1 μmol/L)单独使用不影响其蛋白表达，rn但可显著逆转17β-雌二醇抑制的GROα蛋白表达，rn显著逆转17β-雌二醇抑制单核细胞系细胞U937细胞rn粘附到HUVEC的作用达.结论：通过内皮细胞上rn雌激素受体α，雌激素可能功能性调节人内皮细胞rn的GROα的表达.%AIM: To study the effect of estrogen on expression of rngrowth-regulated oncogene α (GROα) in human umbilical rnvein endothelial cells (HUVEC) in vitro. METHrnODS: Expressions of CXC chemokine GROα mRNA and rnprotein were measured by Northern blotting assay and rnELISA, respectively. The physiological significance of rnGROα expression was tested by static cell adhesion assay.rnRESULTS: Both the GROα mRNA and protein levels rndecreased markedly after HUVEC were exposured to rn17β-estradiol (E2) 0.05 μmol/L. Moreover, the inhibirntion of the protein was depended on the concentration of rn17β-estradiol. Tamoxifen (0.1 μmol/L), an estrogen rnreceptor α antagonist, alone did not affect GROα protein rnexpression, but can reverse the E2-induced inhibition of rnGROα protein expression (by up to 50 % ) and the rnbinding of U937 cells to E2-treated HUVEC (by up to rn40 % ). CONCLUSION: Estrogen might functionally rndown-regulates GROα expression through estrogen rnreceptor α on endothelial cells.