莨菪亭对PC3细胞增殖和凋亡的影响

摘要

AIM: To investigate the effect of scopoletin on cell proliferation and apoptosis of PC3 cells. METHODS:Cell growth curve, MTr assay, and acid phosphatase activity (ACP) were used to determine cell proliferation.Coomassie brillient blue assay was used to measure the content of protein in cells. Light microscope, transmission electronmicroscope, and fluorescence microscope were used to observe scopoletin-induced morphological changes. Apoptosis rate and cell cycle distribution were determined by flow cytometry. RESULTS: The IC 50 ofscopoletin for inhibiting PC3, PAA, and Hela cell proliferation was ( 157 ± 25), ( 154 ± 51 ), and (294 ±100) mg/L, respectively. Scopoletin induced a marked time- and concentration-dependent inhibition of PC3 cell proliferation. Scopoletin reduced the protein content and decreased the ACP level in PC3 cells in a concentrationdependent manner. Cells treated by scopoletin showed typical morphologic changes of apoptosis by light microscope, fluorescence microscope, and transmission electronmicroscope. Apoptosis rate was 0.3 %,2.1%, 9.3 % and 35 % for scopoletin 0, 100, 200,and 400 mg/L, respectively, and cells in G2 phase decreased markedly after being treated with scopoletin.CONCLUSION: Scopoletin inhibited PC3 proliferation by inducing apoptosis of PC3 cells.%目的：研究莨菪亭对人前列腺癌细胞PC3增殖的作用和莨菪亭是否能引起PC3细胞的凋亡.方法：用细胞生长曲线，MTT试验和酸性磷酸酶(ACP)活性来测定细胞增殖，考马斯亮蓝法测细胞内蛋白质的含量，光镜、透射电镜和荧光显微镜观察莨菪亭引起的形态学变化.用荧光显微镜和流式细胞仪确定凋亡率和细胞的周期分布.结果：莨菪亭对PC3，PAA和Hela细胞的IC50分别为(157±25)，(154±51)和(294±100)mg/L，莨菪亭时间和浓度依赖性地抑制PC3细胞的增殖，并引起细胞内蛋白质含量减少和ACP活性降低.经莨菪亭处理后，在光镜、透射电镜和荧光显微镜下可观察到莨菪亭引起的典型的凋亡形态学变化，流式细胞仪测定显示经莨菪亭0，100，200和400 mg/L处理后PC3细胞的凋亡率分别为0.3％，2.1％，9.3％和35％，G2期细胞显著减少.结论：莨菪亭抑制PC3细胞增殖且可引起PC3细胞的凋亡.