乳糖化重组人生长激素在小鼠体内的药物动力学

摘要

AIM: To study the pharmacokinetic characteristics of lactosaminated recombinant human growth hormone (hGH-L) in mice. METHODS: The biodistribution was studied with in vivo radioactive tracer technique.The pharmacokinetics was investigated by radioimmunoassy (RIA) method of hGH-L. The results were compared with that of recombinant human growth hormone (hGH). RESULTS: 125I-hGH-L has remarkable livertaxis. The area under drug concentration-time curve (32686.9 μg· min· L-1) in blood and serum mean residence time (21.4 min) of hGH-L are less than that of hGH (36913.1 μg· min · L-1 and 24.9 min) ( P ＜0.05). In target organ liver, hGH-L distribution half life (1.8 min) and elimination half life (11.1 min) are shorter than that of hGH (2.1 min and 27.7 min) ( P ＜0.05). The area under drug concentration-time curve ( 17621.9 μg· min· L- 1 ) of hGH-L is bigger than that of hGH( 12148.2 μg· min· L- 1 ) ( P ＜ 0.05 ) in liver.CONCLUSION: The pharmacokinetic paramters of hGH-L has obvious advantage over that of hGH.%目的：研究乳糖化基因重组人生长激素(hGH-L)在小鼠体内的药代动力学特征.方法：用放射性核素体内示踪技术研究体内分布.建立hGH-L放射免疫分析(RIA)方法，研究其药代动力学特征，并对比研究基因重组人生长激素(hGH).结果：125I-hGH-L具有明显的趋势肝性.hGH-L的血药时曲线下面积和在血清的平均驻留时间均小于hGH，P＜0.01；而靶器官肝脏的hGH-L分布半衰期、消除半衰期小于hGH，P＜0.05，其药时曲线下面积大于hGH，P＜0.05.结论：hGH-L的药代动力学特征明显优于hGH.