Na-alginate was selected as an entrapping agent to immobilize ammonia oxidizing bacteria in this experiment, designed to explore optimal entrapping condition and to compare immobilized bacteria with non immobilized bacteria in ammonium removal efficiency. Results show that when Na-alginate was at 4%, and CaCl_2 at 3%, an optimal entrapping condition was achieved with bacteria entrapment reaching at 3.0 g L~(-1), and ammonia removing rate at 80%. Compared with non-immobilized cells, the immobilized ones were more adaptable to variation of temperature and pH, as well as exposure to organic matter and toxicants%以海藻酸钠作为氨氧化细菌的包埋固定载体,以固定化氨氧化细菌对氨氮的去除率为主要指标,研究氨氧化细菌的最佳包埋条件,并比较了未固定细胞与固定化细胞两种处理对水中氨氮去除效果的影响.结果表明,当海藻酸钠浓度为4%、CaCl_2浓度为3%、包菌量为3.0 g L~(-1)时,氨气去除率均超过80%,达到最佳包埋条件.与未固定细胞的处理相比,固定化细胞对温度、pH的变化以及有机物、有毒物质污染等环境变化表现出更强的适应能力.
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