通过设定不同的氮(N)、磷(P)浓度一次性培养东海原甲藻,研究了该藻的生长状况及不同生长阶段单位细胞DNA,RNA及蛋白质含量的变化,结果表明,不同氮磷浓度对东海原甲藻的生长产生显著影响,最终生物量与初始的氮磷浓度呈正相关,不同培养组的最大生长率存在显著差异(P＜0.05)；DNA含量与培养液中氮浓度相关性极显著(P＜0.01),但与磷浓度相关性不显著(P＞0.05)；RNA含量与氮磷浓度均呈极显著相关(P＜0.01),而且在不同的生长阶段其含量也会发生变化,随着培养时间的延长,各培养组的RNA含量显著下降(P＜0.05)；RNA/DNA比值与氮磷浓度均显著相关(P＜0.05),而且与生长率呈线性相关关系.培养液中氮浓度显著影响到东海原甲藻单位细胞总蛋白含量,磷缺乏也会使总蛋白含量显著降低.%Changes in growth, cellular DNA, RNA and total protein content related to nitrogen(N) and phosphorus(P) concentrations were studied in a marine dinoflagellate Prorocentrum donghaiense Lu grown in batch cultures. The resalts show that the growth of P. Donghaiense is significantly affected by N and P concentrations. The ultimate biomass depended on the initial N and P concentrations. The maximum growth varied distinctly under different N or P treatments(P<0. 05). DNA content had significant correlation with N concentrations in the cultures but not with P. RNA content was strongly associated with both N and P concentrations(P<0. 01). Different growth stages could also change cellular RNA content. The RNA content decreased along with the extension of experimental time. RNA/DNA ratio had significant correlation with the N and P concentrations. There appeared to be a linear relationship between the growth rate and the ratio of RNA/DNA. Cellular total protein content was significantly affected by N concentrations. P deficiency could also reduce cellular protein content.
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